sgRNA Library Design _{Design from Escherichia coli IAI39}

Chromosome and plasmid (press ctrl or command for selecting multiple fragments):

Design Target:	CDS	RNA
Off Threshold:	The off target penalty threshold, sgRNAs with potential off-target site carrying penalty score lower than the threshold will be eliminated. For the detailed description of the scoring method, please check our paper. Briefly, we suggest off threshold >= 20 for library design. In situations where more sgRNAs are desired, the threshold can be decreased to 10, where the off-target effect of CRISPRi is still very slight as previously reported.	GC Content:	Min: % The minimal GC content of spacer region (percentage, positive integer between 0~100 is accepted).	Max: % The maximum GC content of spacer region (percentage, positive integer between 0~100 and > GC content Min is accepted).
Spacer Length:		Strand:	Template whether the sgRNA is designed targeting (binding) to the template or nontemplate stand of a coding gene. It is suggested by previous reports that dCas9 based CRISPRi system used in this work exhibits (better activity when targeting to non-template strand in the coding region).	Nontemplatewhether the sgRNA is designed targeting (binding) to the template or nontemplate stand of a coding gene. It is suggested by previous reports that dCas9 based CRISPRi system used in this work exhibits (better activity when targeting to non-template strand in the coding region).
NC Design:	No	Yes	NC RNA numbers(1-500):
Email report:	No	Yes	Email:

sgRNA Library Design Design from Escherichia coli IAI39