sgRNA Library Design Design from Customised Input

Annotation File: Accepted file formats: .ptt, .rnt, .gff and .gff3.
Note that the .ptt file is only for CDS target design; and the .rnt file is only for RNA target design.
Design Target: CDS RNA
Genome File: Accepted file formats: .fna and .fasta.
Contig option: added contig:
Off Threshold: The off target penalty threshold, sgRNAs with potential off-target site carrying penalty score lower than the threshold will be eliminated. For the detailed description of the scoring method, please check our paper. Briefly, we suggest off threshold >= 20 for library design. In situations where more sgRNAs are desired, the threshold can be decreased to 10, where the off-target effect of CRISPRi is still very slight as previously reported. GC Content: Min:  % The minimal GC content of spacer region (percentage, positive integer between 0~100 is accepted). Max:  % The maximum GC content of spacer region (percentage, positive integer between 0~100 and > GC content Min is accepted).
Spacer Length: Strand: Template whether the sgRNA is designed targeting (binding) to the template or nontemplate stand of a coding gene. It is suggested by previous reports that dCas9 based CRISPRi system used in this work exhibits (better activity when targeting to non-template strand in the coding region). Nontemplatewhether the sgRNA is designed targeting (binding) to the template or nontemplate stand of a coding gene. It is suggested by previous reports that dCas9 based CRISPRi system used in this work exhibits (better activity when targeting to non-template strand in the coding region).
NC Design: No Yes NC RNA numbers(1-500):
Email report: No Yes Email: